Construction and identification of a specific expression vector for SV40 large T antigen in gastric parietal cell;
SV40T抗原胃壁細胞特異性表達載體的構建與鑒定
Expression and sgnificance of T antigen in gastric carcinoma patients;
T抗原在胃癌組織中的表達及臨床意義
OBJECTIVE To design and construct eukaryocyte expression vector of SV40 virus large T antigen and induce its targeted expression in eukaryocyte.
目的設計、構建SV40病毒T抗原真核表達載體,并導入真核細胞進行表達鑒定。
Methods We investigated the presence of SV40 T antigen genome in 195 blood specimens from wild monkeys(71 from Nin Lang county,60 from Jin Dong county in Yunnan province) and 64 from caged monkeys by means of the polymerae chain reaction(PCR) followed by sequencing.
目的建立猴外周血單核細胞SV40 DNA的PCR檢測方法,對獼猴SV40 T抗原基因進行檢測。